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lrg2 1t vector  (Addgene inc)


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    Structured Review

    Addgene inc lrg2 1t vector
    Lrg2 1t Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lrg2+1t+vector/LRG2%2E1+(Plasmid+%23108098)/bio_rxiv__64898__2026__01__27__702006-334-13-15
    Average 94 stars, based on 83 article reviews
    lrg2 1t vector - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Construct:

    Article Title: Precision CRISPR annotation of the functional enhancer landscape in primary human T cells
    Article Snippet: .. To construct reporter assay vectors, the EFS promoter upstream of GFP in the LRG2.1T vector (Addgene #108098) was replaced by a minimal TATA box promoter. ..

    Reporter Assay:

    Article Title: Precision CRISPR annotation of the functional enhancer landscape in primary human T cells
    Article Snippet: .. To construct reporter assay vectors, the EFS promoter upstream of GFP in the LRG2.1T vector (Addgene #108098) was replaced by a minimal TATA box promoter. ..

    Plasmid Preparation:

    Article Title: Precision CRISPR annotation of the functional enhancer landscape in primary human T cells
    Article Snippet: .. To construct reporter assay vectors, the EFS promoter upstream of GFP in the LRG2.1T vector (Addgene #108098) was replaced by a minimal TATA box promoter. ..

    Article Title: Myo-differentiation reporter screen reveals NF-Y as an activator of PAX3-FOXO1 in rhabdomyosarcoma
    Article Snippet: .. For competition assays, the DNA oligos of the sgRNAs were cloned into LRG2.1T vector (Addgene #108098) using a BsmBI restriction site. .. For all other assays where individual gene knockouts were performed, the sgRNAs were cloned into LR2.1Tpuro vector (modified from Addgene #108098; GFP replaced by puromycin resistance through in-fusion).

    Clone Assay:

    Article Title: Myo-differentiation reporter screen reveals NF-Y as an activator of PAX3-FOXO1 in rhabdomyosarcoma
    Article Snippet: .. For competition assays, the DNA oligos of the sgRNAs were cloned into LRG2.1T vector (Addgene #108098) using a BsmBI restriction site. .. For all other assays where individual gene knockouts were performed, the sgRNAs were cloned into LR2.1Tpuro vector (modified from Addgene #108098; GFP replaced by puromycin resistance through in-fusion).



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